Research Program Summary January 2014 – March 2020

Microsurgery Research Laboratory

Maria Siemionow, MD, PhD, DSc

From January 2014 to March 2020 the Microsurgery Research Laboratory of the Department of Orthopaedics at the University of Illinois at Chicago conducted activities in educational, basic and clinical research areas as well as provided hands-on microsurgery training under 40hrs CME approved Basic and Advanced Microsurgery Courses.

The Microsurgery Research Laboratory has extensively focused on the topics of novel technologies for enhancement of peripheral nerve regeneration, stem cell-based strategies for tolerance induction in Vascular Composite Allotransplantation (VCA) and stem cell-based therapies targeting Duchenne Muscular Dystrophy and the development of new microsurgical training and experimental models.

Dr. Maria Siemionow, fellow researchers and students presented scientific findings in the form of over 90 oral and poster presentations at national meetings and 30 oral and poster presentations at international meetings. Results of the conducted projects have been published in the form of 35 research manuscripts or reports and 14 review articles were published in the high impact scientific journals.

Furthermore, the academic activities of Microsurgery Research Laboratory included the submission of 25 grant applications. Within the last 5 years our laboratory has been awarded six grants funded by the American Heart Association, Uiversity of Illinois at Chicago Chancellor’s Fund Proof of Concept Award Program (UIC POC) and the Armed Forces Institute of Regenerative Medicine (AFIRM) funded by the US Department of Defense.

In years 2015 the syllabus for the Basic and Advanced Microsurgery Training Courses were designed and approved by the UIC CME Office to grant 40 hours of CME credit.

Since then 35 surgical residents and fellows from the United States, Germany and Brazil have completed both the

Basic and /or Advanced Microsurgery Courses.

Microsurgery Research Laboratory is currently working on projects supported by The Armed Forces Institute of Regenerative Medicine (AFIRM)/Department of Defense US funding (Projects CTA-01 and CTA-04) to develop chimeric cells therapies for tolerance induction. In 2016, Biotechnology Sponsor funding was granted for development of novel chimeric cell based therapy for treatment of Duchenne Muscular Dystrophy and this studus are progressing from pre-clinical feasibility and functional studies towards clinical application.

Abstracts and Presentations  

 

Since January 2014 research studies and results of Microsurgery Research have been presented at 82 national and 30 international scientific and clinical meetings held by acknowledged scientific associations and societies in the area of transplantation, microsurgery, plastic surgery, and regenerative medicine.

 

      

2014

1.     Bobkiewicz A, Uygur HS, Kwiecien G, Madajka M, Siemionow M: Epineural Sheath Jacket as a New Surgical Technique for Neuroma Prevention in the Rat Sciatic Nerve Model: A Preliminary Report (e-Poster), AAHS Annual Meeting, Kauai, Hawaii, USA, January 8-11, 2014.

 

2.     Kwiecien G, Uygur HS, Bobkiewicz A, Madajka M, Caplan A, Siemionow M: Nerve Gap Repair with Human Epineural Sheath Conduit Supported with Human Mesenchymal Stem Cells (oral presentation), ASPN Annual Meeting, Kauai, Hawaii, USA, January 10-12, 2014.

 

3.     Madajka M, Ozturk C, Uygur HS, Lukaszuk M, Siemionow M: Characteristics of the Human Epineural Sheath Properties for Enhancement of Nerve Regeneration – a Preliminary Report (oral presentation), ASPN Annual Meeting, Kauai, Hawaii, USA, January 10-12, 2014.

 

4.     Uygur HS, Kwiecien G, Bobkiewicz A, Madajka M, Siemionow M: Epineural Sheath Tube Prevents Fat Graft Absorption – a Preliminary Report (e-Poster), ASPN Annual Meeting, Kauai, Hawaii, USA, January 10-12, 2014.

 

5.     Bobkiewicz A, Kwiecien G, Uygur HS, Madajka M, Siemionow M: Human Epineural Sheath Conduit as an Allograft Alternative for Peripheral Nerve Repair: Anatomy and Feasibility Study in Sheep Model (e-Poster), ASPN Annual Meeting, Kauai, HI, January 10-12, 2014.

 

6.     Cwykiel J, Kwiecien G, Askar M, Siemionow M:   Migratory Pathways of Human Ex-vivo Created Di-chimeric Cells in the Athymic Nude Rat Model: A Preliminary Report (oral presentation), ASRM Annual Meeting, Kauai, Hawaii, USA, January 11-14, 2014.

 

7.     Uygur HS, Ozturk C, Bozkurt M, Kwiecien G, Madajka M, Siemionow M: A New Vascularized Cervical Lymph Node Tranplantation Model: an Anatomic Study in Rat (oral presentation), ASRM Annual Meeting, Kauai, Hawaii, USA, January 11-14, 2014.

 

8.     Madajka M, Ozturk C, Szopinski J, Uygur HS, Kwiecien G, Bobkiewicz A, Siemionow V, Siemionow M: Long Nerve Defects Repair with Epineural Sheath Conduit – Large Animal Model (poster), ASRM Annual Meeting, Kauai, Hawaii, USA, January 11-14, 2014.

 

9.     Uygur HS, Ozturk C, Kwiecien G, Djohan R, Siemionow M: A New Large Animal Hemi-facial Transplantation Medel: an Anatomic Cadaver Study in Sheep (poster), ASRM Annual Meeting, Kauai, HI, January 11-14, 2014.

 

10.  Kwiecien G, Cwykiel J, Madajka M, Bobkiewicz A, Uygur S, Siemionow M: Donor-Recipient Chimeric Cell Tranplantation as a Novel Rescue Therapy for Acute Radiation Syndrome: A Preliminary Report (oral presentation), PSRC 59th Annual Meeting, New York, New York, USA, March 6-9, 2014.

 

11.  Bobkiewicz A, Uygur H, Kwiecien G, Madajka M, Siemionow M: Epidural Sheath Jacket as a New Surgical Technique for Neuroman Prevention in the Rat Sciatic Nerve Model: A Preliminary Report (oral presentation), PSRC 59th Annual Meeting, New York, New York, USA, March 6-9, 2014.

 

12.  Uygur S, Kwiecien G, Bobkiewicz A, Madajka M, Siemionow M: Application of Epineural Sheath For Fat Graft Volume Maintenance (oral presentation), American Association of Plastic Surgeons 93rd Annual Meeting, Miami, Florida, USA, April 5-8, 2014.

 

13.  Kwiecien G, Cwykiel J, Madajka M, Bobkiewicz A, Uygur S, Siemionow M: Donor-Recipient Chimeric Cell Transplantation as a Novel Rescue Therapy for Acute Radiation Syndrome: A Preliminary Report (oral presentation), American Association of Plastic Surgeons 93rd Annual Meeting, Miami, Florida, USA, April 5-8, 2014.

 

14.  Siemionow M: Cellular Therapies in Face Transplantations (oral presentation), 6th Congress of Modern Oncology, Poznan, Poland, March 27-29, 2014.

 

15.  Bobkiewicz A, Uygur S, Kwiecien G, Madajka M, Siemionow M: Epineural Sheath Jacket as a New Surgical Technique for Neuroma Prevention in the Rat Sciatic Nerve Model (poster presentation), FESSH 2014, Paris, France, June 18-21, 2014.

 

16.  Siemionow M, Kwiencien G, Madajka M, Uygur S, Bobkiewicz A, Caplan A: Functional Results of Peripheral Nerve Repair with Human Epineural Sheath Conduit Supported with Human Mesenchymal Stem Cells (poster presentation), World Transplantation Congress, San Francisco, California, USA, June 26-31, 2014.

 

17.  Cwykiel J, Kwiencien G, Askar M, Siemionow M:   The Potential of Ex-Vivo Created Human Di-Chimeric Cells for Migration and Engraftment in the Nude Rate Model (poster presentation), World Transplantation Congress, San Francisco, California, USA, June 26-31, 2014.

 

18.  Siemionow M: Human Epineural Sheath Conduit Augmented with Human Mesenchymal Stem Cells as a New Biologic Construct Supporting Peripheral Nerve Regeneration: A Preliminary Report (oral presentation), Plastic Surgery 2014, Chicago, Illinois, USA, October 10 – 14, 2014.

 

19.  Siemionow M: Advances in Immunosupression (oral presentation), American College of Surgeons Clinical Congress, San Francisco, California, USA, October 26 – 30, 2014.

 

20.  Cwykiel J, Askar M, Kwiencien G, Siemionow M: Migratory Pathways of Human Cord Blood Derived Ex-vivo Created Di-Chimeric Cells in the Nude Rat Model (poster presentation), 4th Biennial Meeting of the American Society for Reconstructive Transplantation, Chicago, IL, USA, November 20-22, 2014.
21.  Cwykiel J, Kwiencien G, Madajka M, Siemionow M: Rescue Therapy of Donor-Recipient Chimeric Cell for an Acute Radiation Syndrome (poster presentation), 4th Biennial Meeting of the American Society for Reconstructive Transplantation, Chicago, IL, USA, November 20-22, 2014.

 

    

2015

 

22.  Bobkiewicz A, Uygur H, Kwiecien G, Maria M, Cwykiel J, Siemionow M: A New Method for Prevention of Neuroma by Epineural Sheath Jacket in the Rat Sciatic Nerve Model (oral presentation), American Society for Peripheral Nerve Annual Meeting, Paradise Island, Bahamas, January 23-25, 2015.

 

23.  Bozkurt M, Zor F, Karagoz H, Uygur H, Cwykiel J, Siemionow M: Effects of Thymus on Donor-Specific Chimerism: Comparison of Osseomyocutaneous Sternum Composite Tissue Allotransplantation Model With and Without Thymus (oral presentation), American Society for Reconstructive Microsurgery Annual Meeting, Paradise Island, Bahamas, January 24-27,

 

24.  Gharb B, Doumit G, Rampazzo A, Papay F, Siemionow M: Dynamic Skeletal Changes of an Osteomyocutaneous Facial Allograft Five Years Following Transplantation (oral presentation), American Society for Reconstructive Microsurgery Annual Meeting, Paradise Island, Bahamas, January 24-27, 2015.

 

25.  Siemionow M, Cwykiel J, Rampazzo A, Gharb B, Madajka M, Bozkurt M, Nasir S, Klimczak A: Presensitization of the VCA Donor with Bone Marrow Transplant of the Recipient Origin Facilitates Chimerism Induction and Extends Vascularized Composite Allograft Survival (oral presentation), American Society for Reconstructive Microsurgery Annual Meeting, Paradise Island, Bahamas, January 24-27, 2015.

 

26.  Cwykiel J, Askar M, Kwiecien G, Siemionow M: Migratory Pathways and Engraftment of Human Cord Blood Derived Ex-vivo Created Di- Chimeric Cells and its Association with Therapeutic Effect on the Allograft Survival (oral presentation), American Association of Plastic Surgeons Annual Meeting, Scottsdale, Arizona, USA, April 11 – 14, 2015.

 

27.  Cwykiel J, Kwiecien G, Madajka M, Bobkiewicz A, Siemionow M: Donor-Recipient Chimeric Cell – A New Bridging Therapy Against Acute Radiation Syndrome (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

28.  Cwykiel J, Kwiecien G, Madajka M, Bobkiewicz A, Karagoz H, Malewski W, Cyran M, Siemionow M: Assessment of Regenerative Potential of Human Epineural Sheath Conduit Supported by Human Mesenchymal Stem Cells Therapy (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

29.  Tfaily E, Cwykiel J, Dastych K, Siemionow M: In Vitro Characterization of Human Hematopoietic Chimeric Cells – a Novel Tolerance Inducing Strategy in Transplantation (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

30.  Cwykiel J, Sklodowska A, Malewski W, Cyran M, Karagoz H, Tfaily E, Askar M, Siemionow M: Phenotype Characterization, Migratory Pathways, Engraftment and Safety Evaluation of Human Cord Blood Derived Ex-Vivo Created Di- Chimeric in NOD SCID Mouse Model: A Preliminary Study (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

31.  Gharb B, Rampazzo A, Doumit G, Papay F, Bernard S, Siemionow M: Dynamic Skeletal Changes of an Osteomyocutaneous Facial Allograft Five Years Following Transplantation (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

32.  Klimczak A, Futoma K, Jundzil A, Patrzalek D, Siemionow M: Assessment of Immunologic, Proangiogenic and Neurogenic Factors of the Human Peripheral Nerve Epineurium for Potential Application in Prevention of Neuroma Formation (oral presentation), International Hand and Composite Tissue Allotransplantation Society 2015 Congress, Philadelphia, Pennsylvania, USA, April 16 – 18, 2015.

 

33.  Cwykiel J, Sklodowska A, Malewski W, Cyran M, Karagoz H, Bryndza E, Askar M, Siemionow M:   Phenotype Characterization, Migratory Pathways, Engraftment and Safety Evaluation of Human Cord Blood Derived Ex-Vivo Created Di- Chimeric in NOD SCID Mouse Model: A Preliminary Study (poster presentation), American Transplant Congress, Philadelphia, Pennsylvania, USA, May 2 – 6, 2015.

 

34.  Tfaily E, Cwykiel J, Dastych K, Sklodowska A, Siemionow M: Human Hematopoietic Chimeric Cells as a Novel Approach for Tolerance Induction After Transplantation (poster presentation), American Transplant Congress, Philadelphia, Pennsylvania, USA, May 2 – 6, 2015.

 

35.  Klimczak A, Futoma K, Jundzil A, Patrzalek D, Siemionow M: Immunologic, Proangiogenic and Neurogenic Assessment of Allogenic Epineurium of the Human Peripheral Nerve for Potential Application in Prevention of Neuroma Formation (poster presentation), American Transplant Congress, Philadelphia, Pennsylvania, USA, May 2 – 6, 2015.

 

36.  Klimczak A, Futoma K, Jundzill A, Patrzalek D, Siemionow M:   Assessment of Immunologic, Proangiogenic and Neurogenic Properties of the Human Peripheral Nerve Epineurium for Potential Application in Prevention of Neuroma Formation (oral presentation), Federation of European Societies for Surgery of the Hand, Milan, Italy, June 17 – 20, 2015.

 

37.  Siemionow M, Madajka M, Uygur S, Oztürk C, Kwiecien G, Szopinski J, Bobkiewicz A, Cwykiel J: Laminin Rich and Immunologically Neutral Epineural Sheath Conduit Supported with Bone Marrow Stromal Cells to Restore Long Nerve Defects (oral presentation), Federation of European Societies for Surgery of the Hand, Milan, Italy, June 17 – 20, 2015.

 

38.  Siemionow M, Kwiecien G, Madajka M, Uygur S, Cwykiel J, Bobkiewicz A, Caplan A: Neuroregenerative and Immunomodulating Properties of Human Epineural Sheath Conduit   Augmented with Human Mesenchymal Stem Cells – a New Biologic Construct Supporting Peripheral Nerve Regeneration (oral presentation), Federation of European Societies for Surgery of the Hand, Milan, ITALY, June 17 – 20, 2015.

 

39.  Siemionow M, Cwykiel J, Rampazzo A, Gharb B, Madajka M, Bozkurt M, Nasir S, Klimczak A: The Reverse Paradigm of Chimerism Induction: Donor Conditioning with Bone Marrow Transfer from the Recipient Facilitates Vascularized Composite Allograft Survival (oral presentation), European Society of Transplantation, Brussels, Belgium, September 13 – 16, 2015.

 

40.  Tfaily E, Cwykiel J, Dastych K, Siemionow M.: Characterization of Bone Marrow Derived CD34+ Ex Vivo Created Human Hematopoietic Chimeric Cells – A Novel Tolerance Inducing Strategy in Transplantation (oral presentation), European Society of Transplantation, Brussels, Belgium, September 13 – 16, 2015.

 

41.  Cwykiel J, Filipek N, Cyran M, Bas C, Strojny M, Tfaily E, Askar M, Siemionow, M.: Phenotype Characterization, Migration, Engraftment and Safety Evaluation of Human Cord Blood Derived EX VIVO Created Di-Chimeric Cells in NOD SCID Mouse Model: A Preliminary Study (oral presentation), European Society of Transplantation,Brussels, Belgium, September 13 – 16, 2015.

 

42.  Siemionow M. How to approach potential VCA donor family. ISODP 2015, South Korea,

 

43.  Cwykiel J, Filipek N, Cyran M, Bas C, Strojny M, Tfaily E, Askar M, Siemionow, M. Evaluation of Phenotype, Migration, Engraftment and Safety of Human Cord Blood Derived Ex-vivo Created Di- Chimeric Cells in NOD SCID Mouse Model: A Preliminary Study (poster presentation), The Transplantation Society, Lorne, Australia, November 11-13, 2015.

 

44.  Tfaily E, Cwykiel J, Dastych K, Siemionow M. Characterization of ex vivo Created Human Hematopoietic Chimeric Cells – a Novel Approach for Tolerance Induction in Transplantation (poster presentation) The Transplantation Society, Lorne, Australia, November 11-13, 2015.

 

45.  Maria Siemionow, Joanna Cwykiel, Marcin Strojny, Rafal Gendek , Malgorzata Cyran Erzsebet Szilagyi , Safak Uygur, Maria Madajka. Regenerative Potential of Human Epineural Sheath Conduit Supported by Human Mesenchymal Stem Cells Therapy. (poster presentation) The Transplantation Society, Lorne, Australia, November 11-13, 2015.

 

46.  Joanna Cwykiel, Natalia Filipek, Malgorzata Cyran, Marcin Strojny, Can Bas, Ewa Bryndza Tfaily, Medhat Askar, Maria Siemionow. Evaluation of Phenotype, Migration, Engraftment and Safety of Human Cord Blood Derived Ex-vivo Created Di- Chimeric Cells in NOD SCID Mouse Model: A Preliminary Study.(oral presentation) IPITA-IXA-CTS Joint Congress, Melbourne, Austarlia, November 15-19, 2015

 

47.  Maria Siemionow, Grzegorz Kwiecien, Joanna Cwykiel, Maria Madajka, Safak Uygur, Marcin Strojny, Adam Bobkiewicz, Arnold Caplan. Application of Human Epineural Sheath Conduit Supported by Human Mesenchymal Stem Cells Therapy for Regeneration of Peripheral Nerve Injuries. (oral presentation) IPITA-IXA-CTS Joint Congress, Melbourne, Austarlia, November 15-19, 2015

 

48.  Joanna Cwykiel, Maria Madajka, Adam Bobkiewicz, Maria Siemionow. Bridging Therapy of Donor-Recipient Chimeric Cell Against Hematopoietic Acute Radiation Syndrome. (poster presentation) IPITA-IXA-CTS Joint Congress, Melbourne, Austarlia, November 15-19, 2015

 

49.  Strojny M, Wojciech M, Szilagyi E, Cyran M, Cwykiel J, Gendek R, Karagoz H, Siemionow M. The Effect of Size Discrepancy of Human Epineural Conduit on the Functional Outcomes of Nerve Regeneration in Athymic Nude Rat Model. A Preliminary Report (poster presentation), American College of Wound Healing and Tissue Repair meeting, Chicago, IL, USA, December 3 – 5, 2015.

 

50.  Marcin Strojny, Wojciech Malewski, Erzsebet Szilagyi, Malgorzata Cyran, Joanna Cwykiel, Rafal Gendek, Huseyin Karagoz, Maria Siemionow. The Influence of Diameter Modification of Human Epieneural Sheath Conduit on the Restoration of Peripheral Nerve Function. A Preliminary Report. American College of Wound Healing and Tissue Repair meeting, Chicago, IL, USA, December 3 – 5, 2015.

 

51.  Maria Siemionow, Joanna Cwykiel, Maria Madajka, Safak Uygur, Can Oztürk, and Adam Bobkiewicz. Restoration of Long Nerve Defects using Epineural Sheath Conduit Supported with Bone Marrow Stromal Cells. American College of Wound Healing and Tissue Repair meeting, Chicago, IL, USA, December 3 – 5, 2015.

 

2016

 

52.  Tfaily E, Cwykiel J, Dastych K, Siemionow M. In Vitro Characterization of the Phenotype, Genotype and Clonogenic Properties of ex vivo Created Human Hematopoietic Chimeric Cells – a Novel Approach for Tolerance Induction in VCA Transplantation (podium scientific presentation), American Society for Reconstructive Microsurgery meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

53.  Cwykiel J, Filipek N, Cyran M, Strojny M, Bas C, Tfaily E, Askar M, Siemionow, M. Migration, Engraftment and Safety of Human Cord Blood Derived Ex-vivo Created Di- Chimeric Cells Therapy tested in NOD SCID Mouse Model: A Preliminary Report (podium scientific presentation), American Society for Reconstructive Microsurgery meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

54.  Lukaszuk M, Cwykiel J, Siemionow, M. Application of Epineural Sheath Conduit Supported with Bone Marrow Stromal Cells for Peripheral Nerve Regeneration and Prevention of Denervation Atrophy in Diabetic Rat Model (poster presentation), AAHS meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

55.  Strojny M, Gendek R, Cwykiel J, Szilagyi E, Cyran M, Malewski W, Karagoz H, Siemionow M. The Effect of Size Discrepancy of Human Epineural Conduit on the Functional Outcomes of Nerve Regeneration in Athymic Nude Rat Model. A Preliminary Report (poster presentation), American Society for Peripheral Nerve meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

56.  Can B, Cwykiel J, Siemionow M. A New Supermicrosurgical Anastomosis Training Model of Saphenous Artery and Great Saphenous Vein for Development of Advanced Microsurgical Skills. A Preliminary Study (abstract presentation), American Society for Reconstructive Microsurgery meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

57.  Zor F, Karogoz H, Cwykiel J, Siemionow M. The Effect of Different Cellular Therapies on Donor-Specific Chimerism and Composite Tissue Allograft Survival in Face Transplantation Model. American Society for Reconstructive Microsurgery meeting, Scottsdale, AZ, USA, January 13 – 19, 2016.

58.  Bryndza Tfaily E, Cwykiel J, Dastych K, Siemionow M: In Vitro Characterization of Phenotype, Genotype, Clonogenic Properties and Tolerogenic Potential of Human Hematopoietic Chimeric Cells – a Novel Strategy for Tolerance Induction in Transplantation (poster presentation), 95th Annual Meeting of the American Association of Plastic Surgeons, New York, USA, May 19-22 2016

59.  Bryndza Tfaily E, Cyran M, Gendek R, Strojny M, Szilagyi E, Cwykiel J, Siemionow M. In vitro and in vivo Characterization of Human Hematopoietic Chimeric Cells – a Novel Approach for Tolerance Induction in Transplantation: An update. American Transplant Congress, Boston, USA, June 11-15, 2016.

60.  Szilagyi E. MD, PhD, Bryndza Tfaily E. PhD, Cwykiel J MS, Siemionow M. Chimeric Cell Transplant for Treatment of Duchenne Muscular Dystrophy: a Preliminary Report. American Transplant Congress, Boston, USA June 11-15, 2016

61.  Strojny M, Gendek R, Cwykiel J, Szilagyi E, Cyran M, Malewski W, Karagoz H, Siemionow M. The Effect of Size Discrepancy of Human Nerve Allograft Derived Epineural Conduit on the Functional Outcomes of Nerve Regeneration in Athymic Nude Rat Model. American Transplant Congress, Boston, USA, June 11-15, 2016

62.  Siemionow M, Strojny M, Malewsk W, Karagoz H, Cyran M. The Impact of Diameter Modification of Human Epineural Sheath Conduits using tissue adhesive on the recovery of 20 mm sciatic nerve gap in Athymic Nude Rat Model: A Preliminary Report. XXI FESSH, Santander, Spain, June, 22-25 2016

63.  Siemionow M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Heydemann A, Bryndza-Tfaily E, Garcia-Martinez J. Chimeric cell transplant for the treatment of Duchenne Muscular Dystrophy. American Society for Reconstructive Transplanation Meeting. Chicago, USA, November, 3-5, 2016

64.  Uygur S, Ozturk C, Bozkurt M, Kwiecien G, Madajka M, Siemionow M. Cervical lymph node transplantation model in rats. ASRT 5th Biennial Meeting, Chicago, IL, November 03-05, 2016.

65.  Siemionow M, Tfaily E. In Vitro and In Vivo Characterization of Human Hematopoietic Chimeric Cells-A Novel Strategy to Induce Tolerance in Transplantation. American Society for Reconstructive Transplanation Meeting. Chicago, USA, November, 3-5, 2016

66.  Cwykiel J, Filipek N, Szilagyi E, Bryndza E, Siemionow M. Assessment of Engraftment and Safety of a New Tolerance Inducing Therapy of Human Cord Blood Derived Ex-vivo Created Di-Chimeric Cells in NOD SCID Mouse Model. American Society for Reconstructive Transplanation Meeting. Chicago, USA, November, 3-5, 2016

67.  Cwykiel J, Filipek BS, Szilagyi E, Bryndza E, Siemionow M. Assessment of Viability, Phenotype and Safety of Cryopreserved Human Cord Blood Derived Ex-vivo Created Di-Chimeric Cells in the NSG Mouse Model: A Preliminary Study. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

68.  Siemionow M, Cwykiel J, Uygur J, Bobkiewicz A, Madajka A, Kwiecien G, Oztürk C, Lukaszuk M, Szopinski J. Epineural Sheath Conduit for Restoration of Long Nerve Defects in a Large Animal Model. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

69.  Tfaily E, Cyran M, Gendek M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Le J, Siemionow M. Tolerogenic Potential and Migratory Pathways of Ex Vivo Created Human Hematopoietic Chimeric Cells – A Novel Strategy to Induce Tolerance in Transplantation. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

70.  CE Bas, Cwykiel J, Siemionow M. Free Vascularized Composite Periosteum Flap Based on the Saphenous Vessels – A New Supermicrosurgical movel for Application in Regenerateive Medicine. A Preliminary Report. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

71.  Siemionow M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Heydemann A, Bryndza-Tfaily E, Garcia-Martinez J. Human Chimeric Cells Technnology of Myoblast and Mensenchymal Stem Cell Origin for Restoration of Muscle Function. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

72.  Bobkiewicz A, Cwykiel J, Uygur S, Francuzik W, Siemionow M. Epineural Sheath Jacket Prevents Neuroma Formation in the Rat Scietic Nerve Model. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

73.  Bobkiewicz A, Cwykiel J, Siemionow M. Study of Anatomic Variations of Brachial and Lumbosacral Plexus Models in a Selected Rat Strains for Application in Nerve Regeneration and Tissue Engineering. ESOT BeST Meeting. Fort Lauderdale, USA, November 10-12, 2016

 

2017

 

74.  Siemionow M, Bobkiewicz A, Cwykiel J, Uygur S, Francuzik W. Epineural sheath jacket prevents neuroma formation in the rat sciatic nerve model. Midwestern Association of Plastic Surgery Annual Meeting, Chicago,Illinois, April 15, 2017.

75.  Uygur S, Siemionow M, Cwykiel J, Bobkiewicz A, Madajka M, Kwiecien G, Ozturk C, Lukaszuk M, Szopinski J. Application of autologous epineural sheath conduit for long nerve defects in a large animal model. Midwestern Association of Plastic Surgery Annual Meeting, Chicago, Illinois, April 15, 2017.

76.  Siemionow M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Heydemann A and Garcia-Martinez J. Transplantation of Chimeric Cells of Myoblast and Mesenchymal Stem Cell Origin for Restoration of Muscle Function. American Transplant Congress, Chicago, USA April 29- May 3, 2017

77.  Cwykiel J, Filipek N, Szilagyi E, Tfaily E, and Siemionow M. Confirmation of the Safety of Cryopreserved Human Cord Blood Derived Di-Chimeric Cells in the NSG Mouse Model. American Transplant Congress, Chicago, USA April 29- May 3, 2017

78.  Siemionow M, Bryndza Tfaily E, Szilagyi E, Cwykiel J, Uygur S, and Czerwinski M. Tolerogenic Potential, Migratory Pathways, Engraftment and Long-term Survival of Human Hematopoietic Chimeric Cells – a Novel Strategy to Induce Tolerance in Transplantation. American Transplant Congress, Chicago, USA April 29- May 3, 2017

79.  Siemionow M, Bobkiewicz A, Cwykiel J, Uygur S, Francuzik W. Application of Epineural Sheath Jacket Prevents Neuroma Formation in the Rat Sciatic Nerve Model. FESSH, Budapest, Hungary, June 21-24 2017

80. Siemionow M, Cwykiel J, Uygur S, Bobkiewicz A, Madajka M, Kwiecien G, Oztürk C, Lukaszuk M, Szopinski J. Restoration of 6-cm Long Nerve Defects with Epineural Sheath Conduit in a Large Animal Model. FESSH, Budapest, Hungary, June 21-24 2017

81.  Cwykiel J, Filipek N, Szilagyi E, Tfaily E, Siemionow M. Confirmation of the Engraftment and Safety of Cryopreserved Human Cord Blood Derived Ex-vivo Created Di- Chimeric Cells in the NSG Mouse Model: A Preliminary Study. FESSH, Budapest, Hungary, June 21-24 2017

82.  Siemionow M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Heydemann A and Garcia-Martinez J. Restoration of Muscle Function with Novel Chimeric Cells Therapy of Myoblast and Mesenchymal Stem Cell Origin. FESSH, Budapest, Hungary, June 21-24 2017

 

2018

 

83.  Siemionow M, Bryndza Tfaily E, Cwykiel J, Uygur S, and Szilagyi E. Comparison of the Engraftment Efficacy of Pro-Tolerogenic Human Hematopoietic Chimeric Cells Following Intravenous and Intraosseous Delivery. American Society for Reconstructive Microsurgery (ASRM) Annual Meeting, Phoenix, Arizona, January 13-16, 2018

84.  Cwykiel J, Rafidi G, and Siemionow M. Tolerance Inducing Therapy of Human Multi-Chimeric Cells for Vascularized Composite Allotransplantation: A Preliminary Study. American Society for Reconstructive Microsurgery (ASRM) Annual Meeting, Phoenix, Arizona, January 13-16, 2018 (presentation)

85.  Garg R, Uygur S, Cwykiel J, Siemionow M. Development of Targeted Muscle Re-innervation model in Hind-limb Amputated Rats” American Society for Peripheral Nerves, Phoenix, Arizona, January 12-14, 2018 (Poster)

86.  Cwykiel J, Rafidi G, and Siemionow M. Tolerance Inducing Therapy of Human Multi-Chimeric Cells for Vascularized Composite Allotransplantation 97th AAPS Annual Meeting ▪ April 7-10, 2018 (poster)

87.  Siemionow M, Cwykiel J, Marchese E, Heydemann A, Garcia-Martinez J, Szilagyi E. A Novel Human Myoblast Chimeric Cells Therapy for Restoration of Muscle Function. Midwestern Association of Plastic Surgery Annual Meeting (MAPS), Illinois, Chicago, April 14, 2018

88.  Cwykiel J, Rafidi G, and Siemionow M. A Novel Universal Multi-Chimeric Cells Therapy for Tolerance Induction in Vascularized Composite Allotransplantation. Midwestern Association of Plastic Surgery Annual Meeting (MAPS), Illinois, Chicago, April 14, 2018

89.  Cwykiel J, Szilagyi E, Rafidi G, and Siemionow M. Novel Tolerogenic HSC/MSC Di-Chimeric Cell Therapy in Vascularized Composite Allograft & Heart Transplant Models: A Preliminary Report. Midwestern Association of Plastic Surgery Annual Meeting (MAPS), Illinois, Chicago, April 14, 2018

90.  Siemionow M, Cwykiel J, Marchese E, Futoma K, Rafidi G, Heydemann A, Garcia-Martinez J, Szilagyi E. A Novel Stem Cell Therapy of Dystrophin Expressing Chimeric Cells of Myoblast and Mesenchymal Stem Cell Origin for Enhancement of Muscle Regeneration and Function. XXIII FESSH, Denmark, Copenhagen, June 13-16, 2018 (presentation)

91.  Cwykiel J, Rafidi G, and Siemionow M. Confirmation of the Feasibility of Ex Vivo Creation of a Novel Human Multi-Chimeric Cells Therapy for Tolerance Induction in Vascularized Composite Allotransplantation. XXIII FESSH, Denmark, Copenhagen, June 13-16, 2018 (presentation)

92.  Siemionow M, Cwykiel J, Marchese E, Futoma K, Rafidi G, Heydemann A, Garcia-Martinez J, Szilagyi E. A Novel Human Myoblast Chimeric Cells Therapy for Restoration of Muscle Function. The Transplantation Society, Spain, Madrid, 2018 June 30-July 5 (presentation)

93.  Cwykiel J, Rafidi G, and Siemionow M. Characterization of Ex Vivo Created Human Multi-Chimeric Cells – A Novel Approach for Tolerance Induction in Vascularized Composite Allotransplantation The Transplantation Society, Spain, Madrid, 2018 June 30-July 5 (poster)

94.  Siemionow M, Cwykiel J, and Rafidi G. Evaluation of Phenotype and Safety of Novel Universal Multi-Chimeric Cells Therapy in the NSG Mouse Model: A Preliminary Study. American Society for Reconstructive Microsurgery; USA, Chicago; November 15-17, 2018 (poster)

95.  M. Siemionow, MD, PhD, MSc, E. Bryndza Tfaily, PhD, K. Futoma, PhD, J. Cwykiel, MS, G. Rafidi, BS, M. Cyran, MD, R. Gendek, MD, PhD and E. Szilagyi, MD, PhD: In Vitro and In Vivo Characterization Of Human Hematopoietic Chimeric Cells. A Novel Strategy To Induce Tolerance In Transplantation. American Society for Reconstructive Microsurgery; USA, Chicago; November 15-17, 2018 (poster)

96.  Siemionow M, MD, PhD, MSc, Cwykiel J, MS, Rafidi G, BS and Szilagyi E, MD, PhD: Characterization of Pro-Tolerogenic Hematopoietic Stem Cell (HSC)/ Mesenchymal Stem Cell (MSC) Di-Chimeric Cell Therapy for Tolerance Induction in Vascularized Composite Allograft and Heart Transplantation: A Preliminary Report. American Society for Reconstructive Microsurgery; USA, Chicago; November 15-17, 2018 (poster).

 

2019

 

97.  Siemionow M. Dystrophin Expressing Chimeric (DEC) Human Cell Therapy for Regeneration of Muscle Components of the the Vascularized Composite Allotransplants. American Society for Reconstructive Microsurgery Annual Meeting. Palm Desert, California, February 1-5, 2019.

98.  Marcin Strojny, MD, Joanna Cwykiel, MS and Maria Siemionow, MD PhD DSc. Human Epineural Sheath Conduits Supported with Human Mesenchymal Stem Cells Enhances Nerve Regeneration in Rat Experimental Model. American Society for Peripheral Nerve (ASPN) 2019 Annual Meeting. Palm Desert, California, Feb 01- 03, 2019.

99.  Siemionow M. Composite Tissue Allotransplantation without Life-Long Immunosuppression. AFIRM II Investigators' meeting, Washington DC, Feb 12 – 13, 2019.

100.                Siemionow M. CTA-01: Human Hematopoietic Chimeric Cells Therapy for Transplantation Tolerance. AFIRM II Investigators' Meeting, Washington DC. Feb 12 – 13, 2019.

101.                Siemionow M. CTA-01: Human Hematopoietic Chimeric Cells Therapy for Transplantation Tolerance.   AFIRM II Investigators' Meeting, Washington DC. Feb 12 – 13, 2019 (poster).

 

 

Manuscripts Published In the years 2014-2019 the results and scientific accomplishments of Microsurgery

Research have been published in 25 research papers or reports and 11 reviews in high

impact scientific journals:

 

2014

 

1.     Ozturk C, Uygur S, Ozturk CN, Lukaszuk M, Siemionow M. Feasibility of using external jugular vein and its branches as Y- and X-shaped vein grafts for bridging of arterial defects and providing additional arterial sources for free flap applications in rat model.     J Reconstr Microsurg. 2014 Jul 30(6):371-4. doi: 10.1055/s-0033-1361930. Epub 2014 Feb 17.

2.     Coffman K, Siemionow M. Ethics of facial transplantation revisited. Curr Opin Organ Transplant. 2014 April 19(2):181-7. doi: 10.1097/MOT.0000000000000058. Review.

3.     Bobkiewicz A, Uygur H, Kwiecień G, Madajka M, Siemionow M. Epineural sheath jacket as a new surgical technique for neuroma prevention in the rat sciatic nerve model: a preliminary report. Plast Reconstr Surg. 2014 Mar;13 (3 Suppl):75. doi: 10.1097/01.prs.0000445097.91845.d5. No abstract available.

4.     Kwiecien G, Cwykiel J, Madajka M, Bobkiewicz A, Uygur S, Siemionow M. Donor-recipient chimeric cell transplantation as a novel rescue therapy for acute radiation syndrome: a preliminary report. Plast Reconstr Surg. 2014 Mar;133(3 Suppl):104. doi: 10.1097/01.prs.0000445123.00363.31. No abstract available

5.     Uygur S, Ozturk C, Kwiecien G, Djohan R, Siemionow M. Sheep hemifacial and auricular transplantation models: an anatomic study. Ann Plast Surg. 2014 Apr;72(4):469-74. doi: 10.1097/SAP.0000000000000122

6.     Konczalik W, Siemionow M. Experimental and clinical methods used for fat volume maintenance after autologous fat grafting. Ann Plast Surg. 2014 Apr;72(4):475-83. doi: 10.1097/SAP.0000000000000117. Review

7.     Doumit G, Gharb BB, Rampazzo A, Papay F, Siemionow M, Zins J.Pediatric vascularized composite allotransplantation. Ann Plast Surg. 2014 Oct;73(4):445-50. doi: 10.1097/SAP.0000000000000300. Review.

 

2015

 

8.     Zor F, Karagoz H, Erdemir AT, Karslioglu Y, Acikel CH, Kapaj R, Guzey S, Gurel MS, Isik S, Siemionow M. Reflectance confocal microscopy as a useful diagnostic tool for monitoring of skin containing vascularized composite allograft rejection: A preliminary study on rats. Microsurgery. 2016 Feb;36(2):144-51. doi: 10.1002/micr.22419. Epub 2015 May 11.

9.     Galdyn I, Swanson E, Gordon C, Kwiecien G, Bena J, Siemionow M, Zins J.Microcirculatory effect of topical vapocoolants. Plast Surg (Oakv). 2015 Summer;23(2):71-6.

10.  Siemionow M. Vascularized composite allotransplantation: a new concept in musculoskeletal regeneration. J Mater Sci Mater Med. 2015 Dec;26(12):266. doi: 10.1007/s10856-015-5601-5. Epub 2015 Oct 27.

11.  Hivelin M, Klimczak A, Cwykiel J, Sonmez E, Nasir S, Gatherwright J, Siemionow M. Immunomodulatory Effects of Different Cellular Therapies of Bone Marrow Origin on Chimerism Induction and Maintenance Across MHC Barriers in a Face Allotransplantation Model. Arch Immunol Ther Exp (Warsz). 2016 Aug;64(4):299-310. doi: 10.1007/s00005-015-0380-8. Epub 2015 Dec 26.

 

2016

 

12.  Kulahci Y, Altuntas SH, Karagoz H, Cwykiel JM, Zor F, Siemionow M. A new total hemiface allotransplantation model in rats. Microsurgery. 2016 Mar;36(3):230-8. doi: 10.1002/micr.22527. Epub 2016 Jan 21

13.  Siemionow M, Rampazzo A, Gharb BB, Cwykiel J, Klimczak A, Madajka M, Nasir S, Bozkurt M.The reversed paradigm of chimerism induction: Donor conditioning with recipient-derived bone marrow cells as a novel approach for tolerance induction in vascularized composite allotransplantation. Microsurgery. 2016 Nov;36(8):676-683. doi: 10.1002/micr.30041. Epub 2016 Mar 14.

14.  Gastman B, Hashem AM, Djohan R, Bernard S, Hendrickson M, Schwarz G, Gharb BB, Rampazzo A, Fernandez A, Zins J, Hoffman GS, Doumit G, Siemionow M, Papay F. Malignant Pyoderma Associated with Granulomatosis with Polyangiitis (Wegener Granulomatosis) as a Unique Indication for Facial Vascularized Composite Allotransplantation: Part I. Plast Reconstr Surg. 2016 Jun;137(6):1007e-15e. doi: 10.1097/PRS.0000000000002162

15.  Bobkiewicz A, Cwykiel J, Siemionow M. Anatomic variations of brachial and lumbosacral plexus models in different rat strains. Microsurgery. 2017 May;37(4):327-333. doi: 10.1002/micr.30078. Epub 2016 Jun 7

16.  Karagoz H, Ozturk S, Siemionow M. Comparison of Neurosensory Assessment Methods in Plastic Surgery. Ann Plast Surg. 2016 Aug;77(2):206-12. doi: 10.1097/SAP.0000000000000855. Review.

17.  MacGillis K, Mejia A, Siemionow M. Hand compression neuropathy: An assessment guide. J Fam Pract. 2016 Jul;65(7):462-71. Review.

18.  Zor F, Meric C, Siemionow M. Effects of hPTPβ inhibitor on microcirculation of rat cremaster muscle flap following ischemia-reperfusion injury.Microsurgery. 2017 Sep;37(6):624-631. doi: 10.1002/micr.30131. Epub 2016 Nov 17.

19.  Siemionow M. The miracle of face transplantation after 10 years. Br Med Bull. 2016 Dec;120(1):5-14. Epub 2016 Nov 23. Review.

 

2017

 

20.  Gharb BB, Rampazzo A, Doumit G, Bernard S, Siemionow M, Papay F, Djohan R.Skeletal Changes of an Osteomyocutaneous Facial Allograft Five Years Following Transplantation. J Craniofac Surg. 2017 Mar;28(2):352-358. doi: 10.1097/SCS.0000000000003292.

21.  Klimczak A, Siemionow M, Futoma K, Jundzill A, Patrzalek D.Assessment of immunologic, proangiogenic and neurogenic properties of human peripheral nerve epineurium for potential clinical application. Histol Histopathol. 2017 Nov;32(11):1197-1205. doi: 10.14670/HH-11-875. Epub 2017 Jan 25

22.  Bassiri Gharb B, Frautschi R, Halasa B, Doumit G, Djohan R, Bernard S, Gastman B, Siemionow M, Papay FA, Rampazzo A. Watershed Areas in Face Transplantation. Plast Reconstr Surg. 2017 Mar;139(3):711-721. doi: 10.1097/PRS.0000000000003130. Review.

23.  Bas CE, Cwykiel J, Siemionow M. A New Supermicrosurgery Training Model of Saphenous Artery and Great Saphenous Vein Anastomosis for Development of Advanced Microsurgical Skills. J Reconstr Microsurg. 2017 Jul;33(6):426-434. doi: 10.1055/s-0037-1599101. Epub 2017 Mar

24.  Siemionow M. The decade of face transplant outcomes. J Mater Sci Mater Med. 2017 May;28(5):64. doi: 10.1007/s10856-017-5873-z. Epub 2017 Mar 16. Review.

25.  Light D, Kundu N, Djohan R, Quintini C, Gandhi N, Gastman BR, Drake R, Siemionow M, Zins JE. Total Abdominal Wall Transplantation: An Anatomical Study and Classification System. Plast Reconstr Surg. 2017 Jun;139(6):1466-1473. doi: 10.1097/PRS.0000000000003327

26.  Siemionow M, Bobkiewicz A, Cwykiel J, Uygur S, Francuzik W.Epineural Sheath Jacket as a New Surgical Technique for Neuroma Prevention in the Rat Sciatic Nerve Model. Ann Plast Surg. 2017 Oct;79(4):377-384. doi: 10.1097/SAP.0000000000001117.

27.  Siemionow M, Uygur S, Madajka M. Application of Epineural Sheath as a Novel Approach for Fat Volume Maintenance. Ann Plast Surg. 2017 Dec;79(6):606-612. doi: 10.1097/SAP.0000000000001176.

 

2018

 

28.  Piechota M, Cywiński J, Piechota A, Kusza K, Siemionow M, Moreno R. Is the unadjusted ICU mortality a good indicator of quality of ICU care? Intensive Care Med. 2018 Jan;44(1):127-128. doi: 10.1007/s00134-017-4903-0. Epub 2017 Aug 25.

29.  Siemionow M, Cwykiel J, Heydemann A, Garcia J, Marchese E, Siemionow K, Szilagyi E. Dystrophin Expressing Chimeric (DEC) Human Cells Provide a Potential Therapy for Duchenne Muscular Dystrophy. Stem Cell Rev. 2018 Jun;14(3):370-384. doi: 10.1007/s12015-018-9807-z.

30.  Siemionow M, Bozkurt M, Zor F, Kulahci Y, Uygur S, Ozturk C, Djohan R, Papay F. A New Composite Eyeball-Periorbital Transplantation Model in Humans: An Anatomical Study in Preparation for Eyeball Transplantation. Plast Reconstr Surg. 2018 Apr;141(4):1011-1018. doi: 10.1097/PRS.0000000000004250.

31.  Garg R, Uygur S, Cwykiel J, Siemionow M. Development of Targeted Muscle Reinnervation Model in Hind Limb Amputated Rats. J Reconstr Microsurg. 2018 Sep;34(7):509-513. doi: 10.1055/s-0038-1639602. Epub 2018 Apr 1.

32.  Kusza K, Mielniczuk M, Krokowicz L, Cywiński JB, Siemionow M. Ringer’s lactate solution enhances the inflammatory response during fluid resuscitation of experimentally induced haemorrhagic shock in rats. Arch Med Sci. 2018 Apr;14(3):655-670. doi: 10.5114/aoms.2017.69771. Epub 2017 Sep 5.

33.  Siemionow M, Cwykiel J, Uygur S, Kwiecien G, Oztürk C, Szopinski J, Madajka M. Application of epineural sheath conduit for restoration of 6-cm long nerve defects in a sheep median nerve model. Microsurgery. 2018 Dec 4. doi: 10.1002/micr.30393. Epub ahead of print

34.  Siemionow M, Cwykiel J, Heydemann A, Garcia-Martinez J, Siemionow K, Szilagyi E Creation of Dystrophin Expressing Chimeric Cells of Myoblast Origin as a Novel Stem Cell Based Therapy for Duchenne Muscular Dystrophy. Stem Cell Rev Rep. 2018 Apr;14(2):189-199

 

 

2019

 

35.  Siemionow M, Bozkurt M, Zor F, Kulahci Y, Uygur S, Ozturk C, Djohan R, Papay F.Reply: A New Composite Eyeball-Periorbital Transplantation Model in Humans: An Anatomical Study in Preparation for Eyeball Transplantation. Plast Reconstr Surg. 2019 Feb;143(2):439e-440e.

36.  Hashem AM, Djohan R, Bernard S, Hendrickson M, Schwarz G, Gharb BB, Rampazzo A, Hoffman GS, Doumit G, Bergfeld W, Zins JE, Siemionow M, Papay F, Gastman B.Face Transplantation for Granulomatosis With Polyangiitis (Wegener Granulomatosis): Technical Considerations, Immunological Aspects, and 3-Year Posttransplant Outcome. Ann Plast Surg. 2019 Mar;82(3):320-329. doi: 10.1097/SAP.0000000000001735

37.  Kulinska KI, Billert M, Sawinski K, Czerniak K, Gaca M, Kusza K, Nowak KW, Siemionow M, Billert H. Local anaesthetics upregulate nitric oxide generation in cord blood and adult human neutrophils. Sci Rep. 2019 Jan 24;9(1):569. doi: 10.1038/s41598-018-37090-9.

38.  Siemionow M, Cwykiel J, Uygur S, Kwiecien G, Oztürk C, Szopinski J, Madajka M. Application of epineural sheath conduit for restoration of 6-cm long nerve defects in a sheep median nerve model. Microsurgery. 2019 May;39(4):332-339. doi: 10.1002/micr.30393. Epub 2018 Dec 4.

39.  Siemionow M, Cwykiel J, Uygur S, Kwiecien G, Oztürk C, Szopinski J, Madajka M Application of epineural sheath conduit for restoration of 6-cm long nerve defects in a sheep median nerve model. Microsurgery. 2019 May;39(4):332-339. doi: 10.1002/micr.30393. Epub 2018 Dec 4.

40.  Domaszewska-Szostek A, Krzyżanowska M, Siemionow M. Cell-Based Therapies for Chronic Wounds Tested in Clinical Studies: Review. Ann Plast Surg. 2019 Dec;83(6):e96-e109. doi: 10.1097/SAP

41.  Siemionow M, Malik M, Langa P, Cwykiel J, Brodowska S, Heydemann A. Cardiac Protection after Systemic Transplant of Dystrophin Expressing Chimeric (DEC) Cells to the mdx Mouse Model of Duchenne Muscular Dystrophy. Stem Cell Rev Rep. 2019 Dec;15(6):827-841.

 

2020

 

42.  Zor F, Bozkurt M, Cwykiel J, Karagoz H, Kulahci Y, Uygur S, Siemionow M, The effect of thymus transplantation on donor-specific chimerism in the rat model of composite osseomusculocutaneous sternum, ribs, thymus, pectoralis muscles, and skin allotransplantation. Microsurgery. 2020 Jan 6 doi:10.1002/micr.30555. [Epub ahead of print]

 

 

 

 

Grant Applications In years 2014-2020 Microsurgery Research Laboratory has submitted 25 research proposals to different grant funding organizations.

 

 

Grant Awards In years 2014-2020 Microsurgery Research has conducted research studies supported by six awards from reputable agencies:

 

             2014

 

1.     Chimeric Cellular Therapy for Bone Marrow Reconstitution in Post-Radiation Syndrome, Defense Advanced Research Projects Agency (DARPA), U.S. Dept. of Defense, 2012-2014, $525,000.

2.     Human Hematopoietic Chimeric Cell Therapy for Transplantation Tolerance, U.S. Army Medical Research / Wake Forest Institute for Regenerative Medicine / AFIRM II, U.S. Dept. of Defense, 2013-2018, $1,500,000.

3.     Composite Tissue Allograft Transplantation without Lifelong Immunosuppression, U.S. Army Medical Research / Wake forest Institute for Regenerative Medicine / AFIRM II, U.S. Dept. of Defense, 2013-2018, $1,500,000.

4.     Novel Human Allograft Epineural Conduit for Restoration of Long Nerve Defect/ Mukoskeletal Transplant Foundation, 2013-2016, $300,000.

 

2015

 

5.     Chimeric Cell Therapy for Treatment of Duchenne Muscular Dystrophy, Chancellor’s Research Award 2015-2016, $35,000.

 

2016

 

6.     Novel Tolerogenic CD34/MSC Di-Chimeric Cell Therapy in Vascularized Composite Allograft and Heart Transplant Models, American Heart Association, 2016-2019, $150,000.

 

Educational Activities In years 2014-2019 Dr. Maria Siemionow has engaged in number of educational activities:

 

1.      Visiting Professor at Cleveland Clinic in Cleveland, OH, 2015

2.       Visiting Professor at UCLA in Los Angeles, CA   2015

3.       Hand Research Opportunities- Ortho Conference 2015

4.       Participated in Medical Student Open House 2015

5.       Moderator for Saturday Conference:  Hand 2015

6.       Participated in Resident Interviews   2015

 

In years 2014-2019 the Microsurgery Research has hosted 17 research fellows and 8 Microsurgery Course participants. At present, the Microsurgery Research scientifically supports two fellowships programs and two undergraduate students are under mentorship.

1.     Basic and Advanced Microsurgery Training Course – Microsurgery Course Trainees:

·       Jason Lee, MD, 2015

·       Isaac Livshetz, MD 2015

·       Joseph Karam, MD 2015

·       Rafael de Fina, MD 2018

·       Michal Harasymczyk, MD 2018

·       Raul-Ciprian Covring, 2018

·       James Heaberlin 2018

·       Klemens Machajewski 2017

2.     Can Emre Bas, MD, Ankara, Turkey 2015

3.     Safak Uygur 2017

4.     Huseyin Karagoz 2015

5.     Martin Strojny, MD, Poznan, Poland 2015

6.     Mentored:  Rafal Gendek, MD, PhD, Bydgoszcz, Poland 2015

7.     Mentored:  Anna Domaszewska-Szostek, PhD, Warsaw, Poland 2016

8.     Mentored:  Michał Czerwiński, Medical Student, Warsaw, Poland 2015

9.     Mentored:  Marcin Morawski, Medical Student, Warsaw, Poland 2015

10.  Mentored:  Marta Krzyżanowska, Medical Student, Poznan, Poland 2015

11.  Mentored: Michal Harasymczuk, MD Medical Resident, Poznan, Poland 2018

12.  Mentored: Damian Grybowski, MD 2018

13.  Mentored: Kinga Zastawna, MD Medical Resident, Poznan, Poland 2018

14.  Mentored: Katarzyna Futoma, MSc, Poland 2017

15.  Mentored: Monika Halas, MD 2017

16.  Mentored: Malgorzata Cyran, Poland 2015

17.  Mentored: Ewa Bryndza-Tfaily, PhD 2015

18.  Mentored: George Rafidi, MSc 2018

19.  Mentored: Camaleigh Jaber, MD, UCLA, USA 2019

20.  Paulina Langa, PhD (present) 2018

21.  Sonia Brodowska, MSc, Krakow, Poland (present)2019

22.  Susan Panek, Undergraduate Student, UIC, Chicago, US (present) 2019

23.  Kristina Perchischena, Undergraduate Student, UIC, Chicago, US (present) 2019

24.  Paulina Langa PhD Research Fellow 2018-present

 

Clinical Activities 1.     Participates in clinical activities and evaluates patients at the Orthopaedics Clinic at University of Illinois at Chicago.

2.     Performs surgical procedures in patients with hand surgery and peripheral nerve surgery problems.

3.     Assesses candidates for human hand transplantation.

 

Other Research Activities 1.     Developed Protocol for Human Upper Extremity Allotransplantation, 2016

2.     Submitted Protocol for Human Upper Extremity Allotransplantation and received IRB approval, 2016

3.     Submitted request and received UNOS approval, 2016

 

Awards

In years 2014-2019 scientific communications and projects ran by Dr. Maria Siemionow at Microsurgery Research have been acknowledged by scientific environment on international and domestic meetings:

 

2014

1.     Great Immigrants Award, Carnegie Foundation of New York, 2014

2015

2.     Cell Transplant Society Scientific Award for Application of Human Epineural Sheath Conduit Supported by Human Mesenchymal Stem Cells Therapy for Regeneration of Peripheral Nerve Injuries at 2015 Joint Congress of the IPITA-IXA-CTS in Melbourne, Australia, 2015

3.     Golden Hipolit Award, Hipolit Cegielski Society, Poznan, Poland

2016

4.     Blue Ribbon Award, Poster Presentation for “Chimeric Cell Transplantation for Treatment of Duchenne Muscular Dystrophy: A Preliminary Report” American Transplant Congress, Boston, MA, 2016

5.     Received Heritage Award granted by the Polish American Congress

2017

Awarded a title of the Business Women of the Year by Polish Foundation

 

2018

6.     Best Presentation Award “A Novel Human Myoblast Chimeric Cells Therapy for Restoration of Muscle function” Midwestern Association of Plastic Surgeons, Chicago, IL, 2018

7.     The Business Women of the Year Award by Polish Business Foundation

         2019

 

           7.   Received   Golden Otis Award